| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Functional Divergence in Shrimp Anti-Lipopolysaccharide Factors (ALFs): From Recognition of Cell Wall Components to Antimicrobial Activity
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| Autores: |
Rosa, Rafael Diego
Vergnes, Agnes
De Lorgeril, Julien
Goncalves, Priscila
Perazzolo, Luciane Maria
Saune, Laure
Romestand, Bernard
Fievet, Julie
Gueguen, Yannick
Bachere, Evelyne
Destoumieux-garzon, Delphine
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| Data: |
2013-07
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| Ano: |
2013
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| Resumo: |
Antilipopolysaccharide factors (ALFs) have been described as highly cationic polypeptides with a broad spectrum of potent antimicrobial activities. In addition, ALFs have been shown to recognize LPS, a major component of the Gram-negative bacteria cell wall, through conserved amino acid residues exposed in the four-stranded beta-sheet of their three dimensional structure. In penaeid shrimp, ALFs form a diverse family of antimicrobial peptides composed by three main variants, classified as ALF Groups A to C. Here, we identified a novel group of ALFs in shrimp (Group D ALFs), which corresponds to anionic polypeptides in which many residues of the LPS binding site are lacking. Both Group B (cationic) and Group D (anionic) shrimp ALFs were produced in a heterologous expression system. Group D ALFs were found to have impaired LPS-binding activities and only limited antimicrobial activity compared to Group B ALFs. Interestingly, all four ALF groups were shown to be simultaneously expressed in an individual shrimp and to follow different patterns of gene expression in response to a microbial infection. Group B was by far the more expressed of the ALF genes. From our results, nucleotide sequence variations in shrimp ALFs result in functional divergence, with significant differences in LPS-binding and antimicrobial activities. To our knowledge, this is the first functional characterization of the sequence diversity found in the ALF family.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00160/27077/25236.pdf
DOI:10.1371/journal.pone.0067937
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| Editor: |
Public Library Science
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| Relação: |
http://archimer.ifremer.fr/doc/00160/27077/
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| Formato: |
application/pdf
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| Fonte: |
Plos One (1932-6203) (Public Library Science), 2013-07 , Vol. 8 , N. 7 , P. -
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| Direitos: |
2013 Rosa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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